Journal
JOURNAL OF ENVIRONMENTAL SCIENCES
Volume 69, Issue -, Pages 85-94Publisher
SCIENCE PRESS
DOI: 10.1016/j.jes.2017.04.017
Keywords
Extracellular polymeric substances; LC-MS/MS; Signal substances; Protease inhibitor; Quorum sensing
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Funding
- National Key Research and Development Program of China [2016YFE0118800]
- Shenzhen Science and Technology Development Funding -Fundamental Research Plan [JCYJ20150331151358156]
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The detection of acyl homoserine lactones (AHLs) in activated sludge is essential for clarifying their function in wastewater treatment processes. An LC-MS/MS method was developed for the detection of AHLs in both the aqueous and solid phases of activated sludge. In addition, the effects of proteases and extracellular polymeric substances (EPS) on the detection of AHLs were evaluated by adding protease inhibitors and extracting EPS, respectively. Recoveries of each AHL were improved by adding 50 mu L of protease inhibitor, and recoveries were also improved from 0 to 56.9% to 24.2%-105.8% by EPS extraction. Applying the developed method to determine the type and concentration of AHLs showed that C-4-HSL, C-6-HSL, C-8-HSL and 3-oxo-C-8-HSL were widely detected in a suspended activated sludge system. The dominant AHL was C-8-HSL, with a highest concentration of 304.3 ng/L. C-4-HSL was mainly distributed in the aqueous phase, whereas C-6-HSL, C-8-HSL and 3-oxo-C-8-HSL were preferentially distributed in the sludge phase. (c) 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
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