Journal
JOURNAL OF ENDOCRINOLOGY
Volume 237, Issue 3, Pages 301-310Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/JOE-18-0093
Keywords
chorionic somatomammotropin; placental lactogen; sheep; placenta; lentivirus; RNA interference; SLC2A1; SLC2A3
Categories
Funding
- Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture (NIFA), USDA NIFA [2012-67015-30215, W3112]
- NIH [HD 090312]
- NIFA [2012-67015-30215, 578837] Funding Source: Federal RePORTER
Ask authors/readers for more resources
Several developmental windows, including placentation, must be negotiated to establish and maintain pregnancy. Impaired placental function can lead to preeclampsia and/or intrauterine growth restriction (IUGR), resulting in increased infant mortality and morbidity. It has been hypothesized that chorionic somatomammotropin (CSH) plays a significant role in fetal development, potentially by modifying maternal and fetal metabolism. Recently, using lentiviral-mediated in vivo RNA interference in sheep, we demonstrated significant reductions in near-term (135 days of gestation; dGA) fetal and placental size, and altered fetal liver gene expression, resulting from CSH deficiency. We sought to examine the impact of CSH deficiency on fetal and placental size earlier in gestation (50 dGA), and to examine placental gene expression at 50 and 135 dGA. At 50 dGA, CSH-deficient pregnancies exhibited a 41% reduction (P <= 0.05) in uterine vein concentrations of CSH, and significant (P <= 0.05) reductions (approximate to 21%) in both fetal body and liver weights. Placentae harvested at 50 and 135 dGA exhibited reductions in IGF1 and IGF2 mRNA concentrations, along with reductions in SLC2A1 and SLC2A3 mRNA. By contrast, mRNA concentrations for various members of the System A, System L and System y(+) amino acid transporter families were not significantly impacted. The IUGR observed at the end of the first-third of gestation indicates that the near-term IUGR reported previously, began early in gestation, and may have in part resulted from deficits in the paracrine action of CSH within the placenta. These results provide further compelling evidence for the importance of CSH in the progression and outcome of pregnancy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available