4.7 Article

Fermentation and aerobic stability of rehydrated corn grain silage treated with different doses of Lactobacillus buchneri or a combination of Lactobacillus plantarum and Pediococcus acidilactici

Journal

JOURNAL OF DAIRY SCIENCE
Volume 101, Issue 5, Pages 4158-4167

Publisher

ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2017-13797

Keywords

fermentation end-product; ruminal in situ DM degradability; prolamin; corn grain

Funding

  1. Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil) [2013/16720-2]
  2. Conselho Nacional de Desenvolvimento Cientifico Tecnologico (CNPq
  3. Brasilia, Brazil)

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We investigated the effects of different types and doses of inoculants for ensiling rehydrated corn grain. Shelled corn was finely ground and rehydrated to 35% moisture. Treatments were as follows: (1) control (no additives); (2) Lactobacillus plantarum and Pediococcus acidilactici (LPPA) at a theoretical application rate of 1 x 10(5) cfu/g; (3) LPPA at 5 x 10(5) cfu/g; (4) LPPA at 1 x 10(6) cfu/g; (5) Lactobacillus buchneri (LB) at 1 x 10(5) cfu/g; (6) LB at 5 x 10(5) cfu/g; and (7) LB at 1 x 10(6) cfu/g. We detected no effect of inoculant dose. Gas losses were greater in silages treated with LB compared with control and LPPA silages. Treating silages with LB reduced the concentrations of lactic acid and ethanol and increased silage pH and concentrations of acetic acid, propionic acid, and 1,2-propanediol. At silo opening, silages treated with LB had higher counts of lactic acid bacteria but lower yeast counts than the control silage. Aerobic stability was greater for silages treated with LB and lower for silages treated with LPPA compared with the control. The LB reduced dry matter (DM) losses during aerobic exposure, whereas LPPA increased them. Prolamin content was lower in silages treated with LB compared with the control, resulting in greater ruminal in situ DM degradability. Inoculating LB to a dose of 1 x 10(5) cfu/g increased aerobic stability and ruminal in situ DM degradability of rehydrated corn grain silage. The addition of LPPA did not alter the fermentation process and worsened the aerobic stability of rehydrated corn grain silage. Further studies are warranted to confirm these conclusions in other corn hybrids, inoculants, and their combinations.

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