Journal
JOURNAL OF COMPARATIVE NEUROLOGY
Volume 526, Issue 12, Pages 1877-1895Publisher
WILEY
DOI: 10.1002/cne.24455
Keywords
Amacrine cell; Calretinin (RRID: AB_2313763); Comparative; GAD65 (RRID: AB_259920); Human; Melanopsin (RRID: AB_2629473); Neuropeptide Y (RRID: AB_260814); NPY; Parvalbumin (RRID: AB_377329); Pig; Retina; Tyrosine hydroxylase (RRID: AB_390204)
Categories
Funding
- Fight for Sight Denmark
- Ojenfonden
- Synoptikfonden
- Grosser Chr. Andersen og hustrus legat
- Danish Biotechnology Center for Cellular Communication
Ask authors/readers for more resources
Neuropeptide Y (NPY) is a peptide neurotransmitter abundantly expressed in the mammalian retina. Since its discovery, NPY has been studied in retinas of several species, but detailed characterization of morphology, cell-type, and connectivity has never been conducted in larger mammals including humans and pigs. As the pig due to size and cellular composition is a well-suited animal for retinal research, we chose to compare the endogenous NPY system of the human retina to that of pigs to support future research in this field. In the present study, using immunohistochemistry, confocal microscopy and 3D reconstructions, we found NPY to be expressed in GABAergic and calretinin-immunoreactive (-ir) amacrine cells of both species as well as parvalbumin-ir amacrine cells of humans. Furthermore, we identified at least two different types of medium- to wide-field NPY-ir amacrine cells. Finally, we detected likely synaptic appositions between the NPY-ir amacrine cells and melanopsin- and nonmelanopsin-ir ganglion cells, GABAergic and dopaminergic amacrine cells, rod bipolar cells, and horizontal cells, suggesting that NPY-ir cells play diverse roles in modulation of both image and non-image forming retinal signaling. These findings extend existing knowledge on NPY and NPY-expressing cells in the human and porcine retina showing a high degree of comparability. The extensive distribution and connectivity of NPY-ir cells described in the present study further highlights the potential importance of NPY signaling in retinal function.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available