Journal
YEAST PROTOCOLS, THIRD EDITION
Volume 1163, Issue -, Pages 33-44Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-4939-0799-1_4
Keywords
Saccharomyces cerevisiae; Transformation; Yeast; Lithium acetate; PEG; Carrier DNA; Frozen competent
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Transformation is essential to many molecular and genetic investigations in the yeast Saccharomyces cerevisiae. Yeast transformation protocols utilizing the LiAc/ssDNA/PEG method are presented. Protocols for various applications are listed including a method for transformation in 96-well microtiter plate format and another for the production of frozen competent yeast cells that can be used at a moment's notice.
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