Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1072, Issue -, Pages 252-258Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2017.11.018
Keywords
Amlodipine; HPLC-MS/MS; Dried blood spots; Guthrie card; Medication adherence
Funding
- Northern Ireland Health and Social Care Research and Development Division [COM/5170/15]
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A sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9 m/z -> 238.1 m/z and 408.9 -> 294.0 m/z were used to quantify and qualify amlodipine, respectively. The method was validated across the concentration range of 0.5-30 ng/mL by assessing specificity, sensitivity, linearity, precision, accuracy, recovery and matrix effect according to the Food and Drug Administration (FDA) guidelines. This method was also validated clinically within a large pharmacoepi-demiological study in which amlodipine blood concentration was determined in patients who had been prescribed this medication.
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