Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1569, Issue -, Pages 222-228Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2018.07.051
Keywords
Microchip capillary electrophoresis; Laser induced fluorescence; Aptamer; Aflatoxin B-1; Ochratoxin A
Funding
- State Key Laboratory of Phytochemistry and Plant Resources in West China [P2015-KF12]
- Science and Technology Service Network Initiative [ZSTH-007]
- West Light Foundation of The Chinese Academy of Sciences
Ask authors/readers for more resources
An aptamer-based microchip capillary electrophoresis coupled with laser induced fluorescence (MCE-LIF) detection method for fast determination of Aflatoxin B-1 (AFB(1)) and Ochratoxin A (OTA) was developed. Aptamers that are specific to these two mycotoxins were first hybridized with their aptamer complementary oligonucleotides. The double strand DNA that comes in contact with mycotoxin-containing environment would be unwound into separate aptamer-mycotoxin complex and aptamer complementary single strand. Different types of oligonucleotides can be separated in MCE and detected under the aid of fluorescent dye SYBR gold in LIF detection unit. Under the optimal conditions, on-chip aptamer-mycotoxin conjugates analysis was achieved within 3 min with extremely low LODs (0.026 ng/mL for AFB(1) and 0.021 ng/mL for OTA). Specificity study indicated that other major mycotoxins would not cross-react with these two aptamers, demonstrating the good selectivity of the proposed method. Quantification of trace AFB(1) and OTA in real food samples was carried out and satisfactory recoveries were obtained. It is demonstrated that this method is fast, facile and specific for Simultaneous determination of trace AFB(1) and OTA from foodstuffs. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available