Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1568, Issue -, Pages 22-28Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2018.07.024
Keywords
Alcohol analysis; Derivatization; Trichloroacetyl isocyanate; Mass spectrometry
Funding
- National Basic Research Program of China [2013CB733600]
- National Nature Science Foundation of China [21390202, U1663227]
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A sensitive LC-MS/MS method was established to quantify diols and glycerol in fermentation broth using trichloroacetyl isocyanate as instantaneous derivatization reagent for monitoring the production of 1,3-propanediol and 2,3-butanediol from the biodiesel biorefinery process. Due to the derivatization reaction was very quickly at room temperature, only 1 min was needed for the reaction process. In addition, both extraction of analytes and evaporation of water were not employed in the analytical procedure. Furthermore, the isotope of chlorine was beneficial for understanding of the secondary mass spectrum and avoiding false positive results. Therefore, much more accurate results of diols and glycerol concentration in fermentation could be obtained even at very low levels for the evaluation of microbial metabolism pathway modification. (C) 2018 Elsevier B.V. All rights reserved.
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