Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1565, Issue -, Pages 96-104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2018.06.040
Keywords
Maleic acid (MA); Chemical probes; Human kidney cell lines (HK-2); Protein-protein interactions; Proteomics
Funding
- Ministry of Science and Technology (Taipei, Taiwan), Taipei, Taiwan [NSC MOST 104-2113-M-037-005-MY2, NSC 102-2632-B-037-001-MY3]
- Kaohsiung Medical University Aim for the Top Universities Grant [KMU-TP103A15, KMU-TP103A16]
- Kaohsiung Municipal Ta-Tung Hospital [KMTTH-104-015]
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Maleic acid (MA), an industrial raw material, was found to be illegally added to edible starch-based food products in Taiwan in 2013, a practice unheard of in most of the world. MA has been associated with renal dysfunction in many experimental animal studies. In this study, we developed chemical probes to investigate protein-protein interactions between MA and renal proteins. In the fabrication of the MA probes, we used silicon dioxide (SiO2) modified with a silanized linker (3-aminopropyl triethoxyslane, APTES) to generate MA with APTES-SiO2 particles. The probes were then incubated with the cell lysates of normal human kidney cell lines (HK-2) and subjected to MS/MS for identifying several MA-related proteins, including nucleophosmin, neutral alpha-glucosidase AB, translocon-associated protein subunit alpha, elongation factor 1-gamma, 60S acidic ribosomal protein PO-like, and heat shock protein (HSP 90-alpha and beta). Based on our findings, we believed that the probe can potentially be used to identify and detect the target proteins and help characterize a network of MA protein-protein interactions. (C) 2018 Elsevier B.V. All rights reserved.
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