Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1561, Issue -, Pages 48-55Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2018.05.041
Keywords
4-Carboxybenzo-18-crown-6 ether; Electrospinning; 96-Well column solid phase extraction; Crown ether-modified resin composite fibers; Urinary catecholamines
Funding
- National Natural Science Foundation of China [81302469, 81330064]
- Tianjin Medical University Science Foundation [2012KYM11]
Ask authors/readers for more resources
In the present study, we developed a simple and high-throughput solid phase extraction (SPE) procedure for selective extraction of catecholamines (CAs) in urine samples. The SPE adsorbents were electro-spun composite fibers functionalized with 4-carboxybenzo-18-crown-6 ether modified XAD resin and polystyrene, which were packed into 96-well columns and used for high-throughput selective extraction of CAs in healthy human urine samples. Moreover, the extraction efficiency of packed-fiber SPE (PFSPE) was examined by high performance liquid chromatography coupled with fluorescence detector. The parameters affecting the extraction efficiency and impurity removal efficiency were optimized, and good linearity ranging from 0.5 to 400 ng/mL was obtained with a low limit of detection (LOD, 0.2-0.5 ng/mL) and a good repeatability (2.7%-3.7%, n= 6). The extraction recoveries of three CAs ranged from 70.5% to 119.5%. Furthermore, stable and reliable results obtained by the fluorescence detector were superior to those obtained by the electrochemical detector. Collectively, PFSPE coupled with 96-well columns was a simple, rapid, selective, high-throughput and cost-efficient method, and the proposed method could be applied in clinical chemistry. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available