Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1536, Issue -, Pages 216-227Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2017.09.041
Keywords
Online HILIC x RPLC; Interfaces; LTQ-Orbitrap mass spectrometry; Phenolic acids; Danshen
Funding
- Macao Science and Technology Development Fund [007/2014/AMJ]
- Research Committee of the University of Macau [MYRG109-ICMS13-LP, MYRG2014-00089-ICMS-QRCM]
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In the present study, a system was developed for the analysis of phenolic acids in Salvia miltiorrhiza using online comprehensive two-dimensional hydrophilic interaction chromatography and reversed-phase liquid chromatography coupled to a DAD detector and hybrid linear ion trap-Orbitrap mass spectrometry (HILIC x RP-DAD-ESI/HRMS/MSn). The system was configured based on the combination of an XBridge Amide column (150 mm x 4.6 mm, 3.5 mu m) and Accucore PFP column (50 mm x 4.6 mm, 2.6 mu m) for the first and second dimensions, respectively. An additional LC pump was used to dilute the eluent from the first dimension to decrease its elution strength in the second dimension. A back-flush trap column was selected as an interface to make up for the loss of efficiency and resolution due to the online dilution. Under the optimized conditions, a total of 196 peaks of polar compounds were successfully separated and detected in Salvia miltiorrhiza using the developed online HILIC x RP system, which exhibited high orthogonality (73%). The online combination of HILIC and RP provides powerful separation capability for the analysis of polar compounds in samples with complex matrices. (C) 2017 Elsevier B.V. All rights reserved.
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