Journal
JOURNAL OF BREAST CANCER
Volume 21, Issue 1, Pages 28-36Publisher
KOREAN BREAST CANCER SOC
DOI: 10.4048/jbc.2018.21.1.28
Keywords
Matrix metalloproteinases; MCF-7 cells; NF-kappa B; PPAR gamma; Transcription factor AP-1
Categories
Funding
- National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [NRF-2013R1A1A2011718]
- Chonbuk National University Hospital Research Fund
- NRF - Korean government [2008-0062279]
- National Research Foundation of Korea [21A20151313046] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
Purpose: Peroxisome proliferator-activated receptor. (PPAR.) is involved in the pathology of numerous diseases including atherosclerosis, diabetes, obesity, and cancer. Matrix metalloproteinases (MMPs) play a significant role in tissue remodeling related to various processes such as morphogenesis, angiogenesis, tissue repair, invasion, and metastasis. We investigated the effects of PPAR. on MMP expression and invasion in breast cancer cells. Methods: MCF-7 cells were cultured and then cell viability was monitored in an MTT assay. Western blotting, gelatin zymography, real-time polymerase chain reaction, and luciferase assays were performed to investigate the effect of the synthetic PPAR. ligand troglitazone on MMP expression. Transcription factor DNA binding was analyzed by electrophoretic mobility shift assay. A Matrigel invasion assay was used to assess the effects of troglitazone on MCF-7 cells. Results: Troglitazone did not affect MCF-7 cell viability. 12-O-tetradecanoylphorbol-13-acetate (TPA) induced MMP-9 expression and invasion in MCF-7 cell. However, these effects were decreased by troglitazone. TPA increased nuclear factor kappa B and activator protein-1 DNA binding, while troglitazone inhibited these effects. The selective PPAR. antagonist GW9662 reversed MMP-9 inhibition by troglitazone in TPA-treated MCF-7 cells. Conclusion: Troglitazone inhibited nuclear factor kappa B and activator protein-1-mediated MMP-9 expression and invasion of MCF-7 cells through a PPAR gamma-dependent mechanism.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available