Journal
JOURNAL OF BIOTECHNOLOGY
Volume 283, Issue -, Pages 51-61Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2018.07.010
Keywords
Bioprocess; Astaxanthin carotenoid; Accelerated solvent extraction; Antioxidants; GRAS solvents
Categories
Funding
- Bio Based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation program [745695]
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Solvent Extraction was tested to extract astaxanthin from Haematococcus pluvialis in red phase (HPR), by investigating effects of solvents, extraction pressure and temperature. Astaxanthin isomers were identified and quantified in the extract. The performances of acetone and ethanol, Generally Recognized As Safe (GRAS) solvents, were explored. Negligible effect of pressure was found, while with increasing extraction temperature astaxanthin recovery increased fill a maximum value, beyond which thermal degradation seemed to be greater than the positive effect of temperature on extraction. Furthermore, to maximize the extraction yield of astaxanthin, mechanical pre-treatment of HPR biomass was carried out and several extraction runs were consecutively performed. Experimental results showed that after the mechanical pre-treatment the astaxanthin recovery strongly increased while a single extraction run of 20 min was sufficient to extract more than 99% of total astaxanthin extracted. After pre-treatment, maximum recovery of about 87% was found for acetone (pressure = 100 bar; temperature = 40 degrees C; total time = 60 min).
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