Journal
JOURNAL OF BIOPHOTONICS
Volume 11, Issue 6, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201700339
Keywords
COX-2; fluorescence; inflammation; molecular imaging; tumor
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Funding
- National Natural Science Foundation of China [81220108012, 61335007, 81371684, 81000666, 81171395, 81328012, 81729002]
- 973 Key Project [2015CB755504]
- Priority Academic Program Development of Jiangsu Higher Education
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Targeting cyclooxygenase-2 (COX-2) for molecular imaging is an attractive approach applicable for its overexpression in inflammation and many malignancies. Herein, for monitoring COX-2, we synthesize a specific COX-2 probe celecoxib-MPA probe (CMP), based on celecoxib and a water-soluble near-infrared dye dye ICG-Der-02 (MPA). Its high affinity for binding to COX-2 is verified by molecular docking, dynamics simulation and inhibition assay. At cellular level, CMP selectively accumulates in cytoplasm of COX-2-positive cells. in vivo assays, probe guided-imaging in inflamed or cancerous tissues confirms that CMP can bind to the locally endogenic COX-2 and exhibit intense fluorescence. Importantly, we further prove the targeting specificity of CMP as the fluorescence is significantly reduced by blocking COX-2 active site through preinjection with celecoxib. The results suggest that the probe CMP, with favorable hydrophilic property, good biocompatibility, long-term observation, excellent targeting ability and optical imaging capability, could serve as a promising probe for real-time monitoring COX-2 in inflammation and tumor.
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