Journal
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
Volume 37, Issue 6, Pages 1464-1476Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/07391102.2018.1461138
Keywords
micronomicin; tobramycin; DNA; ethidium bromide; groove binding
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Funding
- Natural Science Foundation of Jilin Province [20170101020JC]
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Two aminoglycosides, micronomicin (MN), and tobramycin (TB), binding with DNA were studied using various spectroscopic techniques including fluorescence, UV-Vis, FT-IR, and CD spectroscopy coupled with relative viscosity and molecular docking. Studies of fluorescence quenching and time-resolved fluorescence spectroscopy all revealed that MN/TB quenching the fluorescence of DNA-EB belonged to static quenching. The binding constants and binding sites were obtained. The values of Delta H, Delta S, and Delta G suggested that van der Waals force or hydrogen bond might be the main binding force. FT-IR and CD spectroscopy revealed that the binding of MN/TB with DNA had an effect on the secondary structure of DNA. Binding mode of MN/TB with DNA was groove binding which was ascertained by viscosity measurements, CD spectroscopy, ionic strength, melting temperature (T-m), contrast experiments with single stranded (ssDNA), and double stranded DNA (dsDNA). Molecular docking analysis further confirmed that the groove binding was more acceptable result.
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