Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 71, Issue 3, Pages 173-184Publisher
SPRINGER
DOI: 10.1007/s10858-018-0172-7
Keywords
Baculovirus; Yeast extract; Algal extract; Deuteration; GPCR; Abelson kinase
Categories
Funding
- Swiss National Science Foundation [31-149927, 31-173089]
- Swiss Cancer League [KFS-3603-02-2015]
- Biozentrum Basel International PhD Program
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Baculovirus-infected insect cells have become a powerful tool to express recombinant proteins for structural and functional studies by NMR spectroscopy. This article provides an introduction into the insect cell/baculovirus expression system and its use for the production of recombinant isotope-labeled proteins. We discuss recent advances in inexpensive isotope-labeling methods using labeled algal or yeast extracts as the amino acid source and give examples of advanced NMR applications for proteins, which have become accessible by this eukaryotic expression host.
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