Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 23, Issue 7, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.23.7.075008
Keywords
photobiomodulation; osteoblast; osteocyte; osteoclast; cell viability; proliferation; differentiation; light dose
Funding
- Ormco, Orange County, California
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The objective of this study was to evaluate the effects of varying light doses on the viability and cellular activity of osteoblasts, osteocytes, and osteoclasts. A light application device was developed to apply 940-nm wavelength light from light-emitting diodes on three cultured cells, MC3T3-E1, MLO-A5, and RANKL-treated RAW264.7 cells. The doses (energy density) on cells were 0, 1, 5, and 7.5 J/cm(2). The corresponding light power densities at the cell site were 0, 1.67, 8.33, and 12.5 mW/cm(2), respectively, and the duration was 10 min. The results showed that the three cell types respond differently to light and their responses were dose dependent. Low-dose treatment (1 J/cm(2)) enhanced osteoblast proliferation, osteoclast differentiation, and osteoclastic bone resorption activity. Osteocyte proliferation was not affected by both low- and high-dose (5 J/cm(2)) treatments. While 1 J/cm(2) did not affect viability of all three cell types, 5 J/cm(2) significantly decreased viability of osteocytes and osteoclasts. Osteoblast viability was negatively impacted by the higher dose (7.5 J/cm(2)). The findings suggest that optimal doses exist for osteoblast and osteoclast, which can stimulate cell activities, and there is a safe dose range for each type of cell tested. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).
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