Novel Carbon Dots Derived from Cirsii Japonici Herba Carbonisata and Their Haemostatic Effect

The purpose of this study was to explore the haemostatic effect of novel carbon dots (CDs) derived from Cirsii Japonici Herba Carbonisata (CJHC). Transmission electron microscopy (TEM), high-resolution TEM, Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, fluorescence spectroscopy, X-ray photoelectron spectroscopy, high performance liquid chromatography and a cell counting kit-8 assay of CJHC-CDs were performed. Then the haemostatic effect of this CJHC-CDs was studied in bleeding mouse models. To explore their related haemostatic mechanism, coagulation parameters and platelets (PLT) were measured. The CJHC-CDs ranged in diameter from 2 to 11 nm and had a quantum yield of 0.63%. They exhibited no toxicity up to concentrations of 5752 mu g/mL. After administration, mice had a significantly shortened bleeding time and the coagulation parameters and PLT significantly increased. These results showed the definite haemostatic effect of CJHC-CDs.