Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 106, Issue 7, Pages 1780-1788Publisher
WILEY
DOI: 10.1002/jbm.a.36381
Keywords
random-orientated PCL nanofibers; aligned-orientated PCL nanofibers; mesenchymal stem cells; 3-D cell culture; canine
Funding
- University of Tennessee's Center of Excellence in Livestock Diseases and Human Health (UTCVM, COE) [R181721352]
- Department of Small Animal Clinical Sciences at the College of Veterinary Medicine at the University of Tennessee [E180120]
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Tissue engineering shows great promise for the treatment of degenerative diseases, including bone repair. Polymer nanofibers provide a three-dimensional (3-D) scaffold for attachment and growth of mesenchymal stem cells. Increasing evidence supports that fiber alignment on scaffolds plays a major role in the viability and differentiation of stem cells. We compared the cell viability of canine adipose tissue-derived mesenchymal stem cells (cADMSCs) cultured in the aligned- (NanoAligned) and random- (NanoECM) oriented polycaprolactone (PCL) nanofiber-coated plates to control polystyrene tissue culture plates using a proliferation assay. Ability of the plates to induce differentiation of cADMSCs into osteocytes, adipocytes, and neurons was evaluated based on expression of the osteocyte markers, COL1A1 and osterix; adipocyte markers PPAR2 and LPL; and neuronal marker nestin using RT-PCR. Proliferation results demonstrated that aligned-oriented PCL nanofiber-coated plates were more suitable substrate for cADMSCs after 7 days in culture compared to random-oriented PCL nanofiber-coated or control plates. Additionally, we demonstrated that both 3-D PCL nanofiber-coated plates were a better scaffold for cADMSCs differentiation into osteocytes compared to control plates. In conclusion, our results confirm that PCL nanofiber is a suitable tissue engineering material for use in regenerative medicine for canine patients in vivo. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1780-1788, 2018.
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