Journal
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Volume 106, Issue 8, Pages 2213-2228Publisher
WILEY
DOI: 10.1002/jbm.a.36428
Keywords
cryogel; bioscaffold; polydopamine; mesenchymal stem cells; regenerative medicine
Funding
- Stanford Nano Shared Facilities (SNSF) [1161726-146-DAARZ]
- National Science Foundation [ECCS-1542152]
- Stanford Neuroscience Microscopy Service [NIH NS069375]
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Cryo-hydrogels (cryogels) are polymer hydrogels formed at sub-zero temperatures. Bioscaffolds created from cryogels have interconnected macropores which allow for cell migration, tissue-ingrowth, unhindered diffusion of solutes and mass transport of therapeutics. In this study, we developed collagen based cryogel bioscaffolds and coated them with polydopamine using a simple two-step technique. Cryogel bioscaffolds were synthesized by collagen crosslinking at -20 degrees C and exhibited a macroporous interconnected architecture with 75%+/- 3% porosity. Two groups of pore sizes were observed: 300 +/- 50 mu m and 30 +/- 10 mu m in diameter. The addition of a polydopamine coating to cryogel bioscaffolds was confirmed using composition analysis. This resulted in a 41%+/- 5% decrease in water uptake, 81%+/- 10% decrease in swelling rate and 12%+/- 3% decrease in their degree of dissolution (p<0.05), with a 48%+/- 2% increase in stiffness and 57%+/- 5% increase in compressive strength (p<0.05). Seeding adipose tissue-derived mesenchymal stem cells (AD-MSCs) into polydopamine coated-cryogel bioscaffolds resulted in cells demonstrating a 52%+/- 4% increase in viability and 33%+/- 3% increase in proliferation when compared to AD-MSCs seeded into uncoated-cryogel bioscaffolds (p<0.05). In summary, our novel polydopamine coated-cryogel bioscaffold represents an efficient and low-cost bioscaffold platform to support MSC therapies. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2213-2228, 2018.
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