Journal
JOURNAL OF BIOCHEMISTRY
Volume 163, Issue 6, Pages 481-488Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvy021
Keywords
baculovirus; monoclonal antibody; N-glycosylation; silkworm; trastuzumab
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Silkworm-baculovirus expression systems are efficient means for the production of recombinant proteins that provide high expression levels and post-translational modifications. Here, we characterized the stability, glycosylation pattern and antibody-dependent cell-mediated cytotoxicity activity of anti-HER2 monoclonal antibodies containing native or glycoengineered mammalian-like N-glycans that were produced by using a silkworm-baculovirus expression system. Compared with a monoclonal antibody produced by using a Chinese hamster ovary cell expression system, the glycoengineered monoclonal antibody had comparable thermal stability and a higher antibody-dependent cell-mediated cytotoxicity activity. These results suggest that silkworm-baculovirus expression systems are next-generation expression systems potentially useful for the cost-effective production of therapeutic antibodies.
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