Journal
GENOME RESEARCH
Volume 26, Issue 2, Pages 192-202Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.198291.115
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Funding
- Agence National de Recherche (ANR Blanc EMPREINTE)
- Institut National du Cancer [INCa_5960, INCa_7889]
- Association pour la Recherche contre le Cancer [ARC SFI20101201555, SFI20121205729]
- Fondation pour la Recherche Medicale [FRM DPM20121125544, DEQ20150331703]
- ITMO Cancer [EPIG201416]
- Agency for International Cancer Research (UK)
- MEDDTL [11-MRES-PNRPE-9-CVS-072]
- LABEX EpiGenMEd
- ATIP-AVENIR program
- EpiGeneSys Network of Excellence
- European Research Council (ERC) [615371]
- French Ministere de la Recherche
- Ligue Contre le Cancer
- European Research Council (ERC) [615371] Funding Source: European Research Council (ERC)
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The extent to which histone modifying enzymes contribute to DNA methylation in mammals remains unclear. Previous studies suggested a link between the lysine methyltransferase EHMT2 (also known as G9A and KMT1C) and DNA methylation in the mouse. Here, we used a model of knockout mice to explore the role of EHMT2 in DNA methylation during mouse embryogenesis. The Ehmt2 gene is expressed in epiblast cells but is dispensable for global DNA methylation in embryogenesis. In contrast, EHMT2 regulates DNA methylation at specific sequences that include CpG-rich promoters of germline-specific genes. These loci are bound by EHMT2 in embryonic cells, are marked by H3K9 dimethylation, and have strongly reduced DNA methylation in Ehmt2(-/-) embryos. EHMT2 also plays a role in the maintenance of germline-derived DNA methylation at one imprinted locus, the Slc38a4 gene. Finally, we show that DNA methylation is instrumental for EHMT2-mediated gene silencing in embryogenesis. Our findings identify EHMT2 as a critical factor that facilitates repressive DNA methylation at specific genomic loci during mammalian development.
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