Journal
JOURNAL OF APPLIED SPECTROSCOPY
Volume 85, Issue 2, Pages 335-340Publisher
SPRINGER
DOI: 10.1007/s10812-018-0653-1
Keywords
absorption spectroscopy; absorption spectrum; netropsin; Hoechst 33258; DNA; poly(rA)-poly(rU); thermodynamic parameters
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Funding
- State Committee for Science of the Ministry of Education and Science of the Republic of Armenia [15T-1F105]
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The interaction of groove binding compounds - peptide antibiotic (polyamide) netropsin and fluorescent dye (bisbenzimidazole) Hoechst 33258 - with the double-stranded DNA and synthetic double-stranded polynucleotide poly(rA)-poly(rU) has been studied by spectrophotometry. Absorption spectra of these ligand complexes with nucleic acids have been obtained. Spectral changes at the complexation of individual ligands with the mentioned nucleic acids reveal the similarity of binding of each of these ligands with both DNA and RNA. Based on the spectroscopic measurements, the binding parameters of netropsin and Hoechst 33258 binding to DNA and poly(rA)-poly(rU) - K and n, as well as the thermodynamic parameters Delta S, Delta G, and Delta H have been determined. It was found that the binding of Hoechst 33258 to both nucleic acids is accompanied by a positive change in enthalpy, while in the case of netropsin the change in enthalpy is negative. Moreover, the contribution of entropy to the formation of the complexes is more pronounced in the case of Hoechst 33258.
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