Journal
GENOME BIOLOGY AND EVOLUTION
Volume 7, Issue 4, Pages 1192-1205Publisher
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evv050
Keywords
transposable elements; repeated DNA; TE analysis; Aedes albopictus; Trinity; bioinformatic pipeline
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Funding
- Agence Nationale de la Recherche (ANR Genemobile)
- Centre National de La Recherche Scientifique
- Institut Universitaire de France
- Federation de Recherche 41 Bio-Environnement et Sante
- French Ministry of Superior Education
- A. albopictus genome sequencing project - Agence Nationale de la Recherche (ANR Immunsymbart)
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Repetitive DNA, including transposable elements (TEs), is found throughout eukaryotic genomes. Annotating and assembling the repeatome during genome-wide analysis often poses a challenge. To address this problem, we present dnaPipeTE-a new bioinformatics pipeline that uses a sample of raw genomic reads. It produces precise estimates of repeated DNA content and TE consensus sequences, as well as the relative ages of TE families. We shows that dnaPipeTE performs well using very low coverage sequencing in different genomes, losing accuracy only with old TE families. We applied this pipeline to the genome of the Asian tiger mosquito Aedes albopictus, an invasive species of human health interest, for which the genome size is estimated to be over 1 Gbp. Using dnaPipeTE, we showed that this species harbors a large (50% of the genome) and potentially active repeatome with an overall TE class and order composition similar to that of Aedes aegypti, the yellow fever mosquito. However, intraorder dynamics show clear distinctions between the two species, with differences at the TE family level. Our pipeline's ability to manage the repeatome annotation problem will make it helpful for new or ongoing assembly projects, and our results will benefit future genomic studies of A. albopictus.
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