Journal
GENOME
Volume 58, Issue 2, Pages 81-90Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2015-0001
Keywords
chicken; microRNA; biogenesis; SNP; CBFB
Funding
- National Natural Science Foundation of China [31201795]
- Science and Technology Cooperation Fund Projects of Henan Province of China [122106000046]
- Ministry of Education Innovation Team Development Plan [IRT1236]
- Postdoctoral Science Foundation of China [2013M531675]
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Polymorphisms in miRNA genes could potentially alter various biological processes by influencing the processing and (or) target selection of miRNAs. The rs14120863 (C > G) mutation, which we characterized in a Gushi-Anka F-2 resource population, resides in the precursor region of miR-1666. Association analysis with chicken carcass and growth traits showed that the SNP was significantly associated with carcass weight, evisceration weight, breast muscle weight, leg muscle weight, and body weight at 8 weeks of age, as well as some body size indexes including shank girth, chest breadth, breast bone length, and body slanting length, in the Gushi-Anka F2 resource population. Quantitative RT-PCR results showed that miR-1666 expression levels in muscle tissues differed within various genotypes. Experiment in DF1 cells further confirmed that the SNP in miR-1666 could significantly alter mature miRNA production. Subsequently, using dual-luciferase report assay, we verified that miR-1666 could perform its function through targeting of the CBFB gene. In conclusion, the SNP in the precursor of miR-1666 could significantly reduce mature miR-1666 production. It may further affect the function of miR-1666 through the target gene CBFB, hence it is associated with chicken growth traits.
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