Journal
JOURNAL OF APPLIED ANIMAL RESEARCH
Volume 46, Issue 1, Pages 967-972Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09712119.2018.1440566
Keywords
Bovine oocytes; cumulus expansion; maturation; viability; developmental competence
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Funding
- CONACYT-Mexico [INFR201501-251729]
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Trypan Blue (TB) and Brilliant Cresyl Blue (BCB) are used to select and deternline viability and competer) of oocytes, however, the effects of corpora lutea on cumulus expansion during in vitro maturation are still undeternlined. Cuniulus-oocytes complexes (COCs) from ovaries with ipsilateral(ICL), contralateral (CCL) and without corpora lutea in either ovary (OCL) were selected by visual examination, TB and BCB staining, and matured in vitro to evaluate cumulus expansion. The overall percentage of visually selected COCs was similar in ICL, CCL and OCL (P>0.05). Proportions of live and mature COCs were similar in all groups (P>0.05). The overall percentage of BCB+ COCs was 70.1%. BCB+ ICL COCs had less cumulus expansion 60.5%) than BCB+ CCL and OCL COCs (75.7 and 71.4%, respectively; P < 0.01). BCB ICL COCs had less cumulus expansion (20.0%) than BCB- CCL and OCL (39.7 and 46.1%; P<0.01). BCB+ CCL and OCL COCs showed the highest cumulus expansion index (PG 0.01). Presence of corpus luteum in the ovary affects negatively cumulus cells expansion. TB and BCB staining facilitate the selection of oocytes with higher degrees of cumulus expansion.
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