Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 73, Issue 6, Pages 1546-1552Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dky032
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Funding
- Institut National de la Sante et de la Recherche Medicale and University Hospital of Nimes
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Objectives: To evaluate the performance of the Accelerate Pheno (TM) system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7h. Methods: A panel of 105 clinical strains previously characterized for the presence of beta-lactamase-encoding genes was tested. Approximately 100 cfu of each isolate was inoculated into sterile blood culture bottles and incubated in a BD BACTEC (TM) FX automated system (Becton Dickinson, USA). Positive blood cultures were subjected to parallel testing using the Accelerate Pheno (TM) system and conventional culture methods [identification of isolated colonies by MALDI-TOF and VITEK (R) 2 system (bioMerieux, France), and AST by disc diffusion and Etest following EUCAST recommendations]. Results: The overall identification agreement between the Accelerate Pheno (TM) system and conventional culture methods was 100% (105/105). The overall categorical agreement between the system and culture-based AST was 94.9% (1169/1232), with rates for minor errors of 4.1% (51/1232), major errors 0.3% (4/1232) and very major errors 0.7% (8/1232). The Accelerate Pheno (TM) system produced AST results indicative of third-generation cephalosporinases (26/26) and carbapenem-resistant strains (52/55). Conclusions: The Accelerate Pheno (TM) system is an accurate, sensitive and easy-to-use test for the rapid identification and AST of MDR GNB in bloodstreaminfections. Given the burden of multidrug resistance, its implementation in the microbiology laboratory could be a useful tool for prompt management of sepsis.
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