Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 29, Pages 7655-7662Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b02012
Keywords
Lactobacillus acidophilus NCFM; surface-layer protein; anti-inflammatory; MAPK; NF-kappa B
Funding
- National Natural Science Foundation of China [31471696]
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The objective of our research was to evaluate the molecular mechanism of the anti-inflammatory effects of surface-layer protein (Slp) derived from Lactobacillus acidophilus NCFM in lipopolysaccharide-induced RAW264.7 cells. Our results presented that Sip, with an apparent size of 46 kDa, attenuated the production of TNF-alpha, IL=1 beta, and reactive oxygen species (ROS), by inhibiting the MAPK and NF-kB signaling pathways. In addition, 10 mu g mL(-1) of Slp significantly inhibited NO and PGE(2) production (P < 0.001) through downregulating the expression levels of iNOS and COX-2 protein. Furthermore, Slp was found to inhibit NF-kB p65 translocation into the nucleus to activate inflammatory gene transcription. These findings suggest that Slp is a potential immune-modulating bioactive protein derived from probiotics and holds promise for use as an additive in functional foods.
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