Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 24, Pages 6188-6195Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b00546
Keywords
upconversion nanoparticles; gold nanoparticle; aptamers; fluorescence resonance energy transfer; turn-on fluoresence sensor; Hg2+
Funding
- National Natural Science Foundation of China [31772063]
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In this study, a turn-on nanosensor for detecting Hg2+ was developed based on the fluorescence resonance energy transfer (FRET) between long-strand aptamers-functionalized upconversion nanoparticles (UCNPs) and short-strand aptamersfunctionalized gold nanoparticles (GNPs). In the absence of Hg2+, FRET between UCNPs and GNPs occurred because of the specific matching between two aptamers, resulting in the fluorescence quenching of UCNPs. In the presence of Hg2+, long stranded aptamers fold back into a hairpin structure due to the stable binding interactions between Hg2+ and thymine, leading to the release of GNPs from UCNPs, resulting in the quenched fluorescence restoration. Under the optimized conditions, the nanosensor achieved a linear detection range of 0.2-20 mu M and a low detection limit (LOD) of 60 nM. Meanwhile, it showed good selectivity and has been applied to detecting Hg2+ in tap water and milk samples with good precision.
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