Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 5, Pages 1233-1241Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b05341
Keywords
Pyropia haitanensis; lipoxygenase; hydroperoxide lyase; volatile organic compounds; aroma
Funding
- NSFC [81370532]
- Major Scientific and Technological Project of Zhejiang Province [2016C02055-6B]
- National Science Foundation of Zhejiang [LY18C190004]
- Ningbo Programs for Science and Technology Development [2017C110026, 2017C10020]
- China Agriculture Research System [CAR-50]
- K.C. Wong Magna Fund of Ningbo University
- 151 talents Project
- Li Dak Sum Marine Biopharmaceutical Development Fund
- National 111 Project of China
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The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg.L-1 yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids. C20:5 was the most suitable substrate among the tested fatty acids. The C8-C9 VOCs were generated in good yields from fatty acids, e.g., 2E-nonenal from C20:4, and 2E,6Z-nonadienal from C20:5. Hydrolyzed oils were also tested as substrates. The reactions mainly generated 2E,4E-pentadienal, 2E-octenal, and 2E,4E-octadienal from hydrolyzed sunflower seed oil, corn oil, and fish oil, respectively. PhLOX showed good stability after storage at 4 degrees C for 2 weeks and broad tolerance to pH and temperature. These desirable properties of PhLOX make it a promising novel biocatalyst for the industrial production of volatile aroma compounds.
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