Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 66, Issue 5, Pages 1157-1166Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b05575
Keywords
phytosterol; beta-cryptoxanthin; eryptosis; hemolysis; oxidative stress
Funding
- Ministerio de Economia y Competitividad (MINECO) [AGL2015-68006-C2-1-R]
- Fondo Europeo de Desarrollo Regional (FEDER), Spain [AGL2015-68006-C2-1-R]
- Generalitat Valenciana (Spain) [ACIF/2015/251, ACIF/2016/449]
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The eryptotic and hemolytic effects of a phytosterol (PS) mixture (beta-sitosterol, campesterol, stigmasterol) or beta-cryptoxanthin (beta-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 mu M) were evaluated. beta-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca2+ influx. Co-incubation of both bioactive compounds protected against beta-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca2+ influx, ROS overproduction and GSH depletion at 75 mu M, and hemolysis at both tBOOH concentrations. beta-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by beta-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.
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