Journal
INTERVIROLOGY
Volume 61, Issue 1, Pages 49-52Publisher
KARGER
DOI: 10.1159/000490568
Keywords
Salivirus; Real-time polymerase chain reaction; Gastroenteritis; RNA extraction; Coinfection
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Funding
- University of Turin [BERM_RIC_LOC_15_06]
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Objective: Human Salivirus (SalV) has been associated with gastroenteritis on all continents. Methods: This paper presents the real-time RT-PCR assay for the detection of SalV in clinical fecal samples collected from 192 hospitalized children with acute gastroenteritis in Piedmont, Italy. Results: The most commonly detected virus was Norovirus geno-group II (GII) (33.8%), followed by Rotavirus (21.3%), Sapovirus (10.9%), Parechovirus (8%), Norovirus GI (6.7%), and Adenovirus (1%). PCR detected SalV in 1 (0.5%) subject. Conclusions: Our data show that the detection rate of SalV in diarrheal children (0.5%) is lower than that observed in other countries, where it is reported in diarrheal children in 8.6-1.2% of patients. (C) 2018 S. Karger AG, Basel
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