Journal
PHYSIOLOGICAL REPORTS
Volume 2, Issue 12, Pages -Publisher
WILEY
DOI: 10.14814/phy2.12162
Keywords
AS160; exercise; glucose uptake; insulin sensitivity; slow-twitch muscle; TBC1D1
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Funding
- Niigata University of Health and Welfare
- Japan Society for the Promotion of Science [25350908]
- Grants-in-Aid for Scientific Research [25350908] Funding Source: KAKEN
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A single bout of exercise can enhance insulin-stimulated glucose uptake in both fast-twitch (type II) and slow-twitch (type I) skeletal muscle for several hours postexercise. Akt substrate of 160 kDa (AS160) is most distal insulin signaling proteins that have been proposed to contribute to the postexercise enhancement of insulin action in fast-twitch muscle. In this study, we examined whether the postexercise increase in insulin action of glucose uptake in slow-twitch muscle is accompanied by increased phosphorylation of AS160 and its paralog TBC1D1. Male Wistar rats (similar to 1-month-old) were exercised on a treadmill for 180 min (9 m/min). Insulin (50 lU/mL)-stimulated glucose uptake was increased at 2 h after cessation of exercise in soleus muscle composed of predominantly slow-twitch fibers. This postexercise increase in insulin action of glucose uptake was accompanied by increased phosphorylation of AS160 (detected by phospho-Thr642 and phospho-Ser588 antibody). On the other hand, prior exercise did not increase phosphorylation of TBC1D1 (detected by phospho-Thr590) at 2 h postexercise. These results suggest the possibility that an enhancement in AS160 phosphorylation but not TBC1D1 phosphorylation is involved with increased postexercise insulin action of glucose uptake in slow-twitch muscle.
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