Rhodanobacter hydrolyticus sp nov., a novel DNA- and tyrosine-hydrolysing gammaproteobacterium isolated from forest soil

A bacterial isolate, designated G-5-5(T),was isolated from forest soil at Kyonggi University. Strain G-5-5(T) was acid-tolerant and alkali-tolerant. Cells were strictly aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-sporeforming, rod-shaped, and yellow-coloured. Strain G-5-5(T) hydrolysed DNA and tyrosine; assimilated D-glucose, maltose, N-acetyl-glucosamine and L-fucose; and tolerated only 0.5% NaCl (w/v). Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-5-5(T) formed a lineage within the family Rhodanobacteraceae and that it grouped with but was distinct from various members of the genus Rhodanobacter. The closest member was Rhodanobacter umsongensis GR24-2(T) (97.8 % sequence similarity). The sole respiratory quinone was Q-8. The major polar lipids of strain G-5-5(T) were phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The major cellular fatty acids were summed feature 9 (iso-C-17:1 omega 9c and/or C-16:0 10-methyl), iso-C-15:0, iso-C-17:0, iso-C-16:0 and anteiso-C-15:0. The DNA G+Ccontent of strain G-5-5(T) was 64.1 mol%. DNA-DNA hybridization relatedness between strain G-5-5(T) and other close members of the genus Rhodanobacter ranged from 1 9 % to 45 %. On the basis of the polyphasic characterization and phylogenetic analyses, strain G-5-5(T) represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter hydrolyticus sp. nov. is proposed. The type strain is G-5-5(T) (=KEMB 9005-533(T)=KACC 19113(T)=NBRC 112685(T)).