Simplicispira suum sp nov., isolated from a dust collector at a pig farm

A novel Gram-stain-negative, strictly aerobic, polar-flagellated and rod-shaped bacterium, designated SC1-8(T), was isolated from a dust collector at a pig farm located in Wanju-gun, Jeollabuk-do, Republic of Korea. The strain grew within a temperature range of 4-37 degrees C (optimum, 28-30 degrees C), at pH 7. 0-9. 0 (pH 7. 0-8. 0) and with 0-2% (w/v) NaCl (0%). Colonies were white-beige, circular and convex after 4 days of incubation on Reasoner's 2A agar. Based on the 16S rRNA gene sequence analysis, strain SC1-8(T) was a member of the genus Simphcispira, revealing the highest sequence similarities to Simphcispira limi EMB325(T) (97. 9 %), Simphcispira psychrophila DSM 11588(T) (97. 4 %), Acidovorax defluvu BSB411(T) (97. 3 %), Simphcispira piscis RSG39(T) (97. 1 %) and Simphcispira metamorpha DSM 1837(T) (97. 0 %). The predominant respiratory quinone was Q-8. The polar lipids were phosphatidylethanolamone, diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids (>10% of the total fatty acids) were composed of C-16: 0 and summed feature 3 (C-16:1 omega 6c and/or C-(16:) (1) omega 7c) The DNA G+C content was 63 3 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain SC1-8(T) is presented as a novel species, for which the name Simphcispira suum sp. nov. is proposed. The type strain is SC1-8(T) (=KACC 19329(T)=NBRC 113111(T)).