Amylibacter kogurei sp nov., a novel marine alphaproteobacterium isolated from the coastal sea surface microlayer of a marine inlet

A novel Gram-negative bacterium, designated 4G11(T), was isolated from the sea surface microlayer of a marine inlet. On the basis of 16S rRNA gene sequence analysis, the strain showed the closest similarity to Amylibacter ulvae KCTC 32465(T) (99.0 %). However, DNA-DNA hybridization values showed low DNA relatedness between strain 4G11(T) and its close phylogenetic neighbours, Amylibacter marinus NBRC 110140(T) (8.0 +/- 0.4 %) and Amylibacter ulvae KCTC 32465(T) (52.9 +/- 0.9 %). Strain 4G11(T) had C-18 : 1, C-16 : 0 and C-18 : 2 as the major fatty acids. The only isoprenoid quinone detected for strain 4G11(T) was ubiquinone-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, one unidentified polar lipid, one unidentified phospholipid and one unidentified aminolipid. The DNA G+C content of strain 4G11(T) was 50.0 mol%. Based on phenotypic and chemotaxonomic characteristics and analysis of the 16S rRNA gene sequence, the novel strain should be assigned to a novel species, for which the name Amylibacter kogurei sp. nov. is proposed. The type strain of Amylibacter kogurei is 4G11(T) (KY463497=KCTC 52506(T)=NBRC 112428(T)).