Corynebacterium hadale sp nov isolated from hadopelagic water of the New Britain Trench

A novel heterotrophic, Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain NBT06-6(T), was isolated from the deep seawater in the New Britain Trench and characterized phylogenetically and phenotypically. Optimal bacterial growth occurred at 35 degrees C (range 22-41 degrees C), at pH 6 (4-8) and with 4% (w/v) NaCl (0-10 %). The strain grew at hydrostatic pressures of 0.1-100 MPa (optimum, 0.1 MPa) at 35 degrees C. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain NBT06-6(T) belonged to the genus Corynebacterium, with the highest sequence similarity (97.9 %) to Corynebacterium imitans, and shared low 16S rRNA gene sequence similarities (<97.0 %) with other type strains. The major respiratory menaquinones were MK-8(H-2) and MK-9(H-2). The polar lipids were diphosphatidyglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified aminoglycolipids and four unidentified glycolipids. The major fatty acids detected were C-18:1 omega 9c, C-16:0 and C-15:0. Strain NBT06-6(T) contained meso-diaminopimelic acid and mycolic acids in its cell wall, and mannose, galactose, glucose, arabinose and ribose as major whole-cell sugars. The G+C content of the genomic DNA was 65.1 mol%. The digital DNA-DNA hybridization value and the average nucleotide identity between strain NBT06-6(T) and C. imitans were 24.5 +/- 2.4 and 81.9 %, respectively. The combined genotypic and phenotypic data indicated that strain NBT06-6(T) represents a novel species of the genus Corynebacterium, for which the name Corynebacterium hadale sp. nov. is proposed, with the type strain NBT06-6(T) (=MCCC 1K03347(T)=DSM 105365(T)).