Streptomyces boninensis sp nov., isolated from soil from a limestone cave in the Ogasawara Islands

Actinomycete strain K11-0400(T) was isolated from a soil sample collected in the Ogasawara Islands (also known as the Bonin Islands), Tokyo, Japan. Mature spore chains of strain K11-0400(T) had more than 20 spores per chain. The strain contained LL-diaminopimelic acid as the diamino acid in whole-cell hydrolysates, and MK-9(H-6) and MK-9(H-4) were the predominant menaquinones. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol, and no diagnostic whole-cell sugar was detected. The G+C content of the genomic DNA was 72 mol%. These morphological and chemical features of strain K11-0400(T) indicated that it belonged to the genus Streptomyces. Strain K11-0400(T) showed the highest 16S rRNA gene sequence similarity to Streptomyces naganishii NBRC 12892(T) (97.58 %). However, the DNA-DNA relatedness value between strain K11-0400(T) and the related strain was below 70 %. Based on morphological, cultural and physiological characteristics, and DNA-DNA relatedness data, strain K11-0400(T) should be classified as a new species of the genus Streptomyces, for which the name Streptomyces boninensis sp. nov. is proposed. The type strain of S. boninensis is K11-0400(T) (= NBRC 113073(T), TBRC 7755(T)).