Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume 68, Issue 6, Pages 1955-1962Publisher
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.002772
Keywords
Pinisolibacter ravus; Alphaproteobacteria; Ancalomicrobiaceae; Ancalomicrobium; pine forest soil; facultatively anaerobic
Categories
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2016R1D1A1A09916982]
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A novel bacterium, designated strain E9(T), was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-sporeforming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0-10.0 (optimum, 6.5-8.5) and at 45 degrees C (optimum, 28-32 degrees C). E9(T) was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that E9(T) formed a lineage within the phylum Proteobacteria that was distinct from various members of the order Rhizobiales, including Ancalomicrobium adetum DSM 4722(T) (94.76 % sequence similarity), 'Nitratireductor lucknowense' IITR-21 (92.72 %), Prosthecomicrobium hirschii 16(T)(92.66 %) and Kaistia soli DSM 19436(T) (92.53 %). The predominant isoprenoid quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl-Nmethylethanolamine. Major cellular fatty acids were summed feature 8 (C-10:1 omega 7c and/or C-10:1 omega 6c), summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), and C-16:0. The DNA G+C content of the type strain was 68.4 mol%. Polyphasic characterization indicated that strain E9(T) represented a novel species in a novel genus within a novel family, for which the name Pinisolibacter ravus gen. nov., sp. nov. is proposed. The type strain of Pinisolibacter ravus is E9(T) (=KEMB 9005-534(T) =KACC 19120(T) =NBRC 112686(T)). A formal allocation of the genus Ancalomicrobium to the family Ancalomicrobiaceae fam. nov. is also proposed.
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