Journal
PHYSICAL BIOLOGY
Volume 11, Issue 6, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1478-3975/11/6/065001
Keywords
extracellular shed vesicle; microvesicle; exosome; cellular communication; cancer microenvironment; oncosome
Categories
Funding
- National Cancer Institutes [U54CA143876]
- National Science Foundation GK-12 Program [0841291]
- Alfred P. Sloan Foundation
- Direct For Education and Human Resources
- Division Of Graduate Education [0841291] Funding Source: National Science Foundation
Ask authors/readers for more resources
Extracellular shed vesicles (ESVs) facilitate a unique mode of cell-cell communication wherein vesicle uptake can induce a change in the recipient cell's state. Despite the intensity of ESV research, currently reported data represent the bulk characterization of concentrated vesicle samples with little attention paid to heterogeneity. ESV populations likely represent diversity in mechanisms of formation, cargo and size. To better understand ESV subpopulations and the signaling cascades implicated in their formation, we characterize ESV size distributions to identify subpopulations in normal and cancerous epithelial cells. We have discovered that cancer cells exhibit bimodal ESV distributions, one small-diameter and another large-diameter population, suggesting that two mechanisms may govern ESV formation, an exosome population and a cancer-specific microvesicle population. Altered glutamine metabolism in cancer is thought to fuel cancer growth but may also support metastatic niche formation through microvesicle production. We describe the role of a glutaminase inhibitor, compound 968, in ESV production. We have discovered that inhibiting glutamine metabolism significantly impairs large-diameter microvesicle production in cancer cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available