Journal
NANO LIFE
Volume 4, Issue 4, Pages -Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S1793984414410189
Keywords
Gene delivery; expression; iron oxide; nanoparticles; gene carrier
Categories
Funding
- Major National Scientific Research Program of China [2014CB932200]
- Agricultural Science and Technology Innovation Program (Nanobiotechnology)
- fund of National Transgenic Major Program of China [2009ZX08010-006B]
- National High Technology Research and Development Program of China (863 Program) [2011AA10A201]
- Basic Scientific Research Fund of National Nonprofit Institutes [BSRF 201108]
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The mechanism of gene delivery and expression is one of the most important concerns raised by the development of gene delivery methods. Limited investigation is performed on how magnetic nanoparticles combine with DNA and deliver gene into mammalian cells. In this context, polyethyleneimine (PEI) coated iron oxide magnetic nanoparticles (MNPs) were used as gene carriers for binding and condensing with plasmid DNA expressing enhanced green fluorescent protein (EGFP). The morphology and structure of MNP-DNA complexes were characterized by transmission electron microscopy (TEM) and atomic force microscopy (AFM). We evidenced that large amounts of DNA wrapped around the surface of MNPs and that the MNPs were physically entrapped by the DNA arranged both horizontally and vertically. EGFP gene was successfully expressed under mediation of an external magnetic field which is necessary to efficiently target EGFP gene to the cells. Fluorescence from EGFP was separately detected in the cell cytoplasm and cell nucleus.
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