Journal
INTERNATIONAL JOURNAL OF PHARMACEUTICS
Volume 547, Issue 1-2, Pages 611-620Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2018.06.041
Keywords
Cancer therapy; Tumor target; iRGD
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Funding
- National Natural Science Foundation of China [31300643, 31370505]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Top-notch Academic Programs Project of Jiangsu Higher Education Institutions (TAPP)
- Fundamental Research Funds for the Central Universities [2632018ZD04]
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Tumor-targeted therapy is an attractive strategy for cancer treatment. Peptide hormone thymosin alpha 1 (T alpha 1) has been used against several diseases, including cancer, but its activity is pleiotropic. Herein, we designed a fusion protein T alpha 1-iRGD by introducing the tumor homing peptide iRGD to T alpha 1. Results show that T alpha 1-iRGD can promote T-cell activation and CD86 expression, thereby exerting better effect and stronger inhibitory against melanoma and lung cancer, respectively, than T alpha 1 in vivo. These effects are indicated by the reduced densities of tumor vessels and T alpha 1-iRGD accumulation in tumors. Moreover, compared with T alpha 1, T alpha 1-iRGD can attach more B16F10 and H460 cells and exhibits significantly better immunomodulatory activity in immunosuppression models induced by hydrocortisone. Circular dichroism spectroscopy and structural analysis results revealed that T alpha 1 and T alpha 1-iRGD both adopted a helical confirmation in the presence of trifluoroethanol, indicating the structural basis of their functions. These findings highlight the vital function of T alpha 1-iRGD in tumor-targeted therapy and suggest that T alpha 1-iRGD is a better antitumor drug than T alpha 1.
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