Qualitative and quantitative analysis of lateral diffusion of drugs in human skin

This study aimed to qualitatively and quantitatively analyze lateral diffusion of drugs in dermatomed human skin. Lateral diffusion of calcein and methylene blue dyes in skin was investigated using confocal laser microscopy, calcein imaging, and histology studies. In in vitro permeation studies, two linear microdialysis probes were inserted into the dermis of untreated, poly lacto-glycolic acid microneedle-treated, and ablative lasertreated skin such that one was in the center of the diffusion area and the other was parallel, at 8mm from the central probe. Skin was mounted on Franz cells, sandwiched between donor containing diclofenac sodium solution and receptor containing phosphate buffered saline, pH 7.4. Qualitative techniques revealed faster lateral diffusion of the dyes in microneedle-treated skin than laser-treated skin. Rate of drug diffusion in the central probe in the microneedle-treated skin (11.8 +/- 2.5 mu g/h) was significantly higher than untreated and lasertreated skin (p < 0.05). Rate of lateral diffusion in untreated group (0.7 +/- 0.1 mu g/h) was significantly lower than microneedle and laser-treated skin (p < 0.05). Overall, in vitro microdialysis was demonstrated as a novel and valuable tool that can be employed for quantitative investigation of rate of vertical and lateral diffusion of drugs in intact and microporated skin.