Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 19, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ijms19061558
Keywords
lymphatic endothelial cells; pannexins; Panx1; lymphangiogenesis; cell invasion; Vascular Endothelial Growth Factor-C (VEGF-C)
Funding
- Ligue Nationale contre le cancer (Comite de la Vienne)
- Ligue Nationale contre le cancer (Comite Deux-Sevres)
- Ligue Nationale contre le cancer (Comite Charente-Maritime)
- Ministere de l'enseignement superieur et de la recherche
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The molecular mechanisms governing the formation of lymphatic vasculature are not yet well understood. Pannexins are transmembrane proteins that form channels which allow for diffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol. The expression and function of pannexins in blood vessels have been studied in the last few decades. Meanwhile, no studies have been conducted to evaluate the role of pannexins during human lymphatic vessel formation. Here we show, using primary human dermal lymphatic endothelial cells (HDLECs), pharmacological tools (probenecid, Brilliant Blue FCF, mimetic peptides [(10)Panx]) and siRNA-mediated knockdown that Pannexin-1 is necessary for capillary tube formation on Matrigel and for VEGF-C-induced invasion. These results newly identify Pannexin-1 as a protein highly expressed in HDLECs and its requirement during in vitro lymphangiogenesis.
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