Journal
GENES & GENOMICS
Volume 38, Issue 5, Pages 397-405Publisher
SPRINGER
DOI: 10.1007/s13258-015-0374-2
Keywords
S. chungbukensis DJ77; Phenanthrene degradation; Proteomic responses; Two-dimensional gel electrophoresis
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Funding
- Bio-industry Technology Development Program for the Ministry for food, Agriculture, Forestry, and Rural Affaires of the Republic of Korea [311007-5]
- National Research Foundation of Korea (NRF) - Korea government (MEST) [2012K1A3A1A11031362]
- Next-Generation BioGreen 21 Program (CABX) Rural Development Administration, Republic of Korea [PJ010001]
- National Research Foundation of Korea [2012K1A3A1A11031362] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Sphingomonas chungbukensis DJ77 is a novel aromatic hydrocarbon-degrading bacterium capable of growing on phenanthrene as its sole source of carbon and energy. In this study, the protein expression profiles of S. chungbukensis DJ77 grown in the presence of phenanthrene were investigated by using two-dimensional gel electrophoresis (2-DE). Among 1000 protein spots visualized by 2-DE, the four proteins (i.e. 4-oxalocrotonate decarboxylase, 2-hydroxy-6-oxo-phenylhexa-2,4-dienoate hydrolase, glutathione S-transferase, and 2,3-dihydroxybiphenyl 1,2-dioxygenase) showing the significant upregulation by phenanthrene were identified by reversed-phase liquid chromatography-tandem mass spectrometry. Evidently, these proteins were involved in the metabolism of aromatic hydrocarbons. This can explain why S. chungbukensis DJ77 shows a significantly higher rate of phenanthrene consumption during the degradation process. The present analysis of proteomic responses and the detailed analysis results will be quite helpful to better understand the global physiology of S. chungbukensis DJ77, as proteome databases for various aromatic hydrocarbon-degrading strains have already been established.
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