Journal
GENES & GENETIC SYSTEMS
Volume 90, Issue 4, Pages 231-235Publisher
GENETICS SOC JAPAN
DOI: 10.1266/ggs.15-00030
Keywords
carpel specification; CRISPR-Cas9; DROOPING LEAF (DL); midrib formation; rice
Funding
- MEXT [23248001, 25113008]
- Grants-in-Aid for Scientific Research [13J05127, 23248001, 25113008] Funding Source: KAKEN
Ask authors/readers for more resources
CRISPR-Cas9 technology, which uses an RNA-guided nuclease, has been developed as an efficient and versatile genome-editing method to induce mutations in genes of interest. To examine the feasibility of this method in developmental studies of a model monocot, rice (Oryza sativa), we introduced the construct gDL-1, which produced a guide RNA targeting the DROOPING LEAF (DL) gene. DL regulates midrib formation in the leaf and carpel specification in the flower. Because loss of function of DL causes the drooping leaf phenotype in regenerated seedlings, the effect of gene disruption should be easily detected. In transgenic plants carrying gDL-1, the DL gene was disrupted at high efficiency: seven out of nine plants examined had bi-allelic mutations. All transgenic plants with the bi-allelic mutation showed the drooping leaf phenotype. Observation of cross sections of the leaf blade clearly indicated that these transgenic plants failed to make midrib structures, and were comparable to the severe dl mutant dl-sup1. Thus, CRISPR-Cas9 technology can be a useful and efficient tool in developmental studies in rice.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available