Journal
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
Volume 40, Issue 5, Pages 577-585Publisher
WILEY
DOI: 10.1111/ijlh.12868
Keywords
digital morphology; flow cytometry; monocyte count; optical microscopy; Sysmex XN
Categories
Funding
- Sysmex Corporation
- Sysmex America Inc.
Ask authors/readers for more resources
IntroductionThis study was aimed to evaluate monocyte counts on Sysmex XN-9000, Sysmex CyFlow Space System, and Sysmex DI60 and compare the performance of these systems with the reference optical microscopy (OM) assessment. MethodsIn all, 55 peripheral blood samples, collected in K(3)EDTA tubes, were analyzed with XN-9000, CyFlow System (FlowDiff1 and 2), DI60, and OM. Within-run imprecision was carried out using normal samples. Data comparison was performed with Passing-Bablok regression and Bland-Altman plots. ResultsThe within-run imprecision of monocyte count on XN, FlowDiff, OM, and DI60 ranged between 1.9% for FlowDiff 2 and 22.1% for DI60. The Passing-Bablok regression analysis of absolute count yielded slopes comprised between 0.93 (FlowDiff2 vs DI60) and 1.21 (DI60 vs OM), whereas the intercepts ranged between -0.002 (FlowDiff 1 vs FlowDiff 2) and 0.13 (FlowDiff1 and 2 vs DI60). Bland-Altman plots in absolute values yielded absolute bias comprised between -0.01x10(9)/L (FlowDiff 1 vs FlowDiff 2; DI60 vs OM) and 0.15x10(9) (XN-module vs DI60). ConclusionThe results of this analytical evaluation suggest that flow cytometry generates monocyte counts suitable for routine clinical use. OM or DI60 analysis may be useful for identifying morphologic abnormalities, but does not achieve a satisfactory level of accuracy for enumerating blood cells types such as monocytes, which are usually very low in peripheral blood.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available