Journal
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume 21, Issue 1, Pages 1541-1559Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2018.1497060
Keywords
Sea cucumber; bioactive peptides; physicochemical properties; hydrophobicity; isoelectric point; Q-TOF mass spectrometry
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Funding
- Ministry of Science, Technology and Innovation (MOSTI) of Malaysia [10-05-ABI-FB 037]
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In the present study, the alcalase-generated proteolysates obtained after 8h of proteolysis of stone fish flesh showed the most potent antioxidant activity in terms of DPPH center dot radical scavenging activity (77.43%, IC50 of 0.5mg/mL), ABTS(center dot) radical scavenging activity (92.73%, IC50 of 0.33mg/mL) and FRAP value (39.2mmol/100mL FeSO4). These proteolysates profiled and characterized as antioxidative peptides. The proteolysates were initially subjected to ultrafiltration using MWCO Spin-X UF. Potent fractions were further characterized based on hydrophobicity using reversed-phase high-performance liquid chromatography (RP-HPLC) and isoelectric point using an OFFGEL isoelectric focusing fractionator. Results indicated that most of the antioxidative peptides found in fractions with a molecular weight (MW) of less than 2 kDa, hydrophilic (hydrophilicity >80%) and basic (pI=9.7). The final purified fraction with the highest antioxidant activity was selected for peptide identification and sequencing using Q-TOF mass spectrometry. A total of four peptides were identified, from which Peptide 1 (GVSGLHID) showed the highest antioxidant activity and this has potential as a novel bioingredient of nutraceuticals and functional foods to promote human health.
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