Journal
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
Volume 21, Issue 1, Pages 1107-1116Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10942912.2018.1485030
Keywords
Red-flesh navel orange Cara Cara; Different regions; Phenolic compounds; UPLC-Q-TOF-ESI-MSn; Antioxidant ability
Categories
Funding
- National Natural Science Foundation of China [31571847]
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Red-flesh navel orange Cara Cara is attractive for customers with its bright red color and has been widely planted in China. In this study, phenolic compounds in Cara Cara harvested from five different regions were identified and quantified by ultra high performance liquid chromatography (UPLC) coupled with Quadrupole-Time of Flight -Electrospray ionization-mass spectrometry (Q-TOF-ESI-MSn). Their antioxidant abilities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorption capacity (ORAC) assays. Twenty phenolics were detected in crude extraction, with hesperidin (29.36-44.06%) as the dominant component, followed by narirutin (13.44-17.03%) and apigenin-6,8-di-C-glucoside (8.28-12.38%). No free phenolic acid was found in crude extracts and three free phenolic acids (FPA) were determined through alkaline hydrolysis, wherein ferulic acid (86.22-90.49%) existed dominantly. Fujian Cara Cara showed the highest total phenolic index (TPI) and total flavonoid index (TFI) values, while the lowest TPI and TFI values were detect in Chongqing Cara Cara. DPPH value showed positive correlation with TPC value (r(2)=0.921), while no correlation was found between ORAC and phenolic contents.
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