Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 284, Issue -, Pages 11-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2018.06.024
Keywords
Aspergillus fiavus; laeA; Proteome; Aflatoicin; Secondary metabolite
Categories
Funding
- National Natural Science Foundation of China [31501575]
- Natural Science Foundation of Henan Province [162300410047, 182300410059]
- Science and Technology Development of Henan Province [162102210191]
- Doctoral Scientific Research Start-up Foundation from Henan University of Technology [2015QNJH06, 2015BS016]
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In Aspergillus flavus, laeA affects cell morphology and contributes to the production of secondary metabolites (SMs) production including aflatoxin, cyclopiazonic acid, and aflatrem. Here, we investigated the function of this transcription factor by performing proteomics analysis of the wild-type (WT) and diaeA mutant growing on corn. Notably, our proteomics profile confirmed the functions of extracellular hydrolases, conidial hydrophobin, and response to oxidative stress during the induction of aflatoxin biosynthesis regulated by laeA. Unexpectedly, deletion of lath resulted in the significant upregulation of the NAD(+)-dependent histone deacetylase sirA involved in silencing SM clusters via chromatin remodeling. Accompanying the chromatin modification, enzymes participating in SM, including aflatoxin and cyclopiazonic acid biosynthesis, were drastically decreased. Another unexpected finding was that enzymes in the recently identified ustiloxin B biosynthesis pathway might be regulated by laeA. These data provided novel insights into the complex regulation of laeA and suggested a potential link between lath deletion, NAD(+)-dependent histone deacetylation, and SM production in A. fiavus.
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